Choline is chemically known as 2-hydroxyethyl-trimethyl ammonium hydroxide and is referred as vitamin B4. Choline chloride is used as an important feed additive in animal species, especially chickens. It has recently been claimed as a rediscovered vitamin & performance promoter in poultry. The role of choline in the prevention of conditions such as perosis and liver enlargement in chicks is already well known. Its nutritional importance has been recognized since 1930 and it is now a common dietary supplement for livestock & poultry. The main function of choline is to act as a lipotropic agent and prevents the abnormal fatty infiltration in liver (Fatty Liver Syndrome) thus ensures proper metabolism of the body & effective utilization of the nutrients. It is also a metabolic essential for building and maintaining cell structure. Moreover, it helps in the formation of an excitatory neurotransmitter-acetylcholine, which is responsible for proper function of the nervous system and maintains its harmony.